A mass spectrometer is a marvel of analytical science, capable of revealing the molecular composition of a substance by measuring the mass-to-charge ratio of ions. This device combines physics, chemistry, and precision engineering to transform invisible molecular structures into interpretable spectra.

The operation of a mass spectrometer begins with ionization, where neutral atoms or molecules are converted into charged particles. Ionization methods vary depending on the sample type — electron impact ionization is common for gases, while electrospray ionization (ESI) and matrix-assisted laser desorption ionization (MALDI) are preferred for biomolecules.

Once ionized, particles enter the acceleration chamber, where they are propelled by an electric field. The ions’ velocities depend on their mass-to-charge ratio; lighter ions move faster than heavier ones. The next stage involves separation — typically within a magnetic or electric field — that deflects ions according to their momentum.

The detector registers ions as they reach the end of the instrument,…